Certain 8-hydroxyquinolines as amoebicides

ABSTRACT

A PROCESS FOR THE TREATMENT OF BILHARZIOSIS, AMOEBIASIS, LEISHMANIASIS, FILARIASIS OR MICROBIAL DIARRHOEA IN HUMAN BEINGS WHICH COMPRISES ADMINISTERING AT LEAST ONE ANTIMONY SALT OF   8-HYDROXYQUINOLINE, 6-METHYL-8-HYDROXYQUINOLINE, 5-FLUORO-8-HYDROXQUINOLINE, 5-CHLORO-8-HYDROXYQUINOLINE, 5-NITRO-8-HYDROXYQUINOLINE, 5-SULPHO-8-HYDROXYQUINOLINE, 5-CHLORO-7-IODO-8-HYDROXYQUINOLINE, 5,7-DIBROMO-8-HYDROXYQUINOLINE, 5-CHLORO-7-IODO-6-METHYL-8-HYDROXYQUINOLINE, 5,7-DIBROMO-6-METHYL-8-HYDROXYQUINOLINE.   AMOEBICIDAL COMPOSITION COMPRISING AT LEAST ONE ANTIMONY SALT OF   8-HYDROXYQUINOLINE, 6-METHYL-8-HYDROXYQUINOLINE, 5-FLUORO-8-HYDROXQUINOLINE, 5-CHLORO-8-HYDROXYQUINOLINE, 5-NITRO-8-HYDROXYQUINOLINE, 5-SULPHO-8-HYDROXYQUINOLINE, 5-CHLORO-7-IODO-8-HYDROXYQUINOLINE, 5,7-DIBROMO-8-HYDROXYQUINOLINE, 5-CHLORO-7-IODO-6-METHYL-8-HYDROXYQUINOLINE OR 5,7-DIBROMO-6-METHYL-8-HYDROXYQUINOLINE   AND A PHARMACEUTICALLY ACCEPTABLE CARRIER.

United States Patent 3,769,409 CERTAIN 8-HYDROXYQUINOLINES AS AMOEBICIDES Eugene Riviere, Issy-les-Moulineaux, Danyele Yvette Vilarel, Chantilly, Roger Lucien Debrie, Brenouille, and Gerard Paul Marie Henri Loiseau, Sceaux, France, assignors to Ugille Kuhlmann, Paris, France No Drawing. Filed Sept. 15, 1970, Ser. No. 72,540 Claims priority, application France, Sept. 19, 1969,

6931912 Int. Cl. A61k 27/00 US. Cl. 424-245 1 Claim ABSTRACT OF THE DISCLOSURE A process for the treatment of bilharziosis, amoebiasis, leishmaniasis, filariasis or microbial diarrhoea in human beings which comprises administering at least one antimony salt of 8-hydroxyquinoline, 6-methyl-S-hydroxyquinoline, 5-fluoro-8-hydroxyquinoline, S-chloro-8-hydroxyquinoline, S-nitro-8-hydroxyquinoline, 5-sulpho-8-hydroxyquinoline, 5-chloro-7-iodo-8-hydroxyquinoline, 5,7-dibromo-8-hydroxyquinolinc, 5-chloro-7-iodo-G-methyl-8-hydroxyquinoline or 5,7-dibromo-6-methyl-8-hydroxyquinoline.

Amoebicidal composition comprising at least one anti- 'mony salt of 8-hydroxyquinoline, 6-methyl-8-hydroxyquinoline, S-fluoro-S-hydroxyquinoline, 5-chloro-8-hydroxyquinoline, S-nitro-8-hydroxyquinoline, S-sulpho-8-hydroxyquinoline, 5-chloro-7-iodo-8-hydroxyquinoline, 5,7-dibromo-8-hydroxyquinoline, 5-chloro-7-iodo-6-methyl-8-hydroxyquinoline or 5 ,7 -dibromo-6-methyl-8-hydroxyquinoline and a pharmaceutically acceptable carrier.

The present invention relates to new medicaments with amoebicidal activity.

According to the invention a process is provided for the treatment of bilha rziosis, amoebiasis, leishmaniasis, filariasis or microbial diarrhoea in human beings which comprises administering at least one antimony salt of 3,769,409 Patented Oct. 30, 1973 ice PREPARATION 1 Antimony salt of 8-hydroxyquinoline A hydrochloric acid solution of antimony chloride is prepared by dissolving 22.9 parts of antimony trichloride in 40 parts of distilled water containing 10 parts by volume of 36% pure hydrochloric acid.

On the other hand, 54.5 parts of 8-hydroxyquinoline hydrochloride are dissolved in 40 parts of distilled Water and the solution is filtered if necessary.

This solution is added to that of antimony chloride, the mixture is heated to 45-50 C. and neutralised by the addition of a 20% solution of ammonia to a pH 6-7. It is agitated for some minutes at a temperature between 50 C. and 60 C. filtered at this temperature and the antimony salt is washed with distilled water containing acetic acid (2 parts of acetic acid to 500 parts of distilled water) until the washings no longer contain chlorides. It is dried at 100 C. and 52 parts of the salt of the above formula are obtained, the contents of hydroxyquinoline and antimony of which are respectively 78.8% and 21.7%.

PREPARATION 2 Antimony salt of 6-methyl-8-hydroxyquinoline 30 N O 3 M01. wt. 595. 8

The process is as in Preparation 1, using 58.7 parts of 6-methyl-8-hydroxyquinoline hydrochloride in place of the 8-hydroxyquinoline hydrochloride. A salt having a content of methylhydroxyquinoline of 79.6% and a content of antimony of 20.8% is obtained.

PREPARATION 3 Antimony salt of 5,7-dibromo-8-hydroxyquinoline bO M01. wt. 439. 8

600 parts by volume of a 92.43% solution of ethyl alcohol and 30.3 parts of 5,7-dibromo-8-hydroxyquinoline are introduced into an apparatus provided with a stirrer, a thermometer and a reflux condenser. The mixture is heated under reflux a solution of 23 parts of antimony trichloride in 50 parts by volume of 36% pure hydrochloric acid is slowly added. The reflux temperature 'is maintained for some minutes in order to obtain a clear solution. The product is filtered at about 60 C. in order to eliminate possible impurities and is neutralised at this temperature by the slow addition of about parts by volume of a 20% solutionof ammonia to pH 6-7. The antimony salt is filtered ofii, washed with a 92.43% solution of ethanol, then with distilled water until the washings no longer contain chlorides, and dried at C. 41.5 parts of a salt with the following contents are obtained: bromine, 35.92%; antimony, 27.17%; nitrogen, 3.17%.

3 PREPARATION 4 Antimony salt of 5,7-dibromo-6-methyl-8- hydroxyquinoline CH: Br NJ I SbO Mo]. wt. 453.8

The preparation of this salt is analogous to the process of Preparation 3 but using 31.7 parts of 5,7-dibromo-6- methyl-8-hydroxyquinoline in place of the 5,7-dibromo-8- hydroxyquinoline. Weight of antimony salt obtained: 43 parts.

Contents of: Percent Nitrogen 3.05 Bromine 34.95 Antimony 27.32

PREPARATION Antimony salt of 5-chloro-7-iodo-8-hydroxyquinoline bO M01. wt. 442.3

The procedure is as in Preparation 3, but 30.5 parts of 5 chloro-7-iodo-8-hydroxyquinoline are used in place of the 5,7-dibromo-8-hydroxyquinoline. Weight of antimony salt obtained: 40 parts.

Contents of: Percent Nitrogen 3 .19 Chlorine 7.99 Iodine 29.39 Antimony 28.

PREPARATION 6 Antimony salt of 5-chloro-7-iodo-6-methyl-8- hydroxyquinoline CH: O

bO M01. wt. 456.3

PREPARATION 7 Antimony salt of 5-chloro-S-hydroxyquinoline Mol. wt. 816. 3

4 The procedure is as described in Preparation 3, but 18 parts of S-chloro-8-hydroxyquinoline are used in place of the 5,7 dibromo-8-hydroxyquinoline. Weight obtained:

29 parts.

Contents of: 1 Percent Chlorine 11.30 Nitrogen 4.53 Antimony 38.85

PREPARATION 8 A hydrochloric acid solution of antimony chloride is prepared by dissolving 23 parts of antimony trichloride in 40 parts of distilled water containing 10 parts by volume of 36% pure hydrochloric acid.

On the other hand 19 par'tsfof 5-nitro-8-hydroxyquinoline are dissolved in 400 parts of distilled water containing parts by volume of 96% pure sulphuric acid.

The clear solution obtained is added to that of the antimony chloride. The mixture is heated to 50-60" C., neutralised by the addition of a 20% solution of ammonia to pH 33.5 and maintained for some minutes with agitation at a temperature between 50 C. and 60 C. The antimony salt formed is filtered off at this temperature, washed in distilled water until the washings no longer contain sulphates and dried at 100 C. Weight obtained: 32 parts.

Content of nitrogen: 8.65%. Content of antimony: 37.12%.

PREPARATION 9 Antimony salt of 5-sulpho-8-hydroxyquinoline S OaNa i 2 Mol. wt. 680.8

(A) 36 parts of neutral sodium tartrate are dissolved in 100 parts of distilled water and there is also prepared a hydrochloric acid solution of antimony chloride by dissolving 23 parts of antimony trichloride in 40 parts of distilled water containing 10 parts by volume of pure 36% hydrochloric acid. This solution is added very slowly to the first solution, the pH of themixture being kept at 'between 7 and 7.5 by the addition of 10% soda, say about 100 parts. If the mixture is not clear it is filtered.

(B) In addition, 44.5 parts of 5-sulpho-8-hydroxyquinoline are dissolved in 500 parts of distilled water con-- taining 25 parts by volume of a 30% solution of sodium hydroxide. The mixture is heated to 80-85 C., the pH of the solution being adjusted to 88.2. If cloudiness is still present, it is filtered.

The solution of the complex antimony tartratepre- 'pared under (A) is slowly introduced with agitation into the hot solution (B). On cooling the antimony salt of the 5-sulpho-8-hydroxyquinoline crystallises. It is filtered 01f, Washed with a 42.5% solution of ethanol, then with a 192.43% solution of ethanol, and finally is dried at 80 C.

Weight obtained: 45 parts.

Content of nitrogen: 4.52%; sulphur: 9.95%; antimony: 19%.

PREPARATION Antimony salt of 5-fluoro-8-hydroxyquinoline SbO M01. wt =299. 7

The solution (A) of S-fluoro-8-hydroxyquinoline hydrochloride is prepared by dissolving at 80 C. 18 parts of S-fluoro-8-hydroxyquinoline in 2000 parts of distilled water containing 18 parts by volume of 36% pure hydrochloric acid.

This solution is then poured with agitation into solution (A) and the mixture, maintained at 50 C., is brought to pH 3.7 to 4 by the addition of 20% ammonia (about 60 parts by volume). The precipitate is filtered, drained and washed with distilled water until the washings no longer contain chlorides. After drying at 100 to 110 C. one obtains 27 parts of the antimony salt of 5- fluoro-8-hydroxyquinoline.

Analysis.-Calculated for C HgFNO Sb (percent): C, 36.05; H, 1.68; N, 4.67; F, 6.33; Sb, 40.60. Found (percent): C, 37.26; H, 2.06; N, 4.78; F, 6.50; Sb, 40.66.

TOXICOLOGICAL PROPERTIES BACTERIOSTAT IC PROPERTIES The antimony salt of S-hydroxyquinoline, chosen by way of example, is suspendedin polyethylene glycol 400. The culture medium is prepared from a peptonized medium treatedwith salt and gelose, having the following composition:

. G" Meat extract 5 Peptone 10 Sodium chloride 5 Gelose 20 Distilled water, pH 7.2, 1000 g. (q.s.).

After liquefaction on a water-bath and cooling to 40 50 C. this medium is run into Petri dishes containing the aforesaid suspension of the antimony salt and is strongly agitated so as to distribute some insoluble grains of the latter as regularly as possible in the gelose-containing medium. The bacterial strains are transferred in peptonized salted broth (Without gelose) the day before the test. After 24 hours in an oven at 37 C. the medium run into a Petri dish is inoculated in streaks on the surface and the dishes are put in the oven at 37 C. A control dish receives the polyethylene glycol 400. The results are asfollows:

Bacteriostatic concentrations expressed in mg./litre after 72 hours of incubation at 37 C.

Staphylococcus 10 E. coli 20 Enterococcus I 10 Pneumobacillus 10 Pyocanic 40 400 used, have always shown a culture positive for the germs used.

AMOEBICIDAL PROPERTIES The tests consisted in determining in vitro the amoebicidal activity on a strain of Entamoeba dysenteriae kept on a Pavlova-Jones medium the reference substance taken being emetine hydrochloride and two different processes being used.

(1) Inhibition at the start of the cultures The minimal concentration which prevents the striking of the cutlure after 72 hours at 37 C. is determined. The inoculation consists of about 10,000 amoebae per tube of culture, and the product to be tested is suspended in Tween 80 (a polyoxyethylenated sorbitol monooleate).

(2) Lethal action on a culture of 3 days The minimal concentration which kills all the amoebae of a culture of 3 days after 48 hours at 37 C. is determined. The inoculation consists of about 10,000 amoebae per tube, and the product to be tested is suspended in Tween 80.

The results obtained are collected in Table I, II and III below. A represents the antimony salt of 8-hydroxyquinoline, B the antimony salts of 6-methyl-8-hydroxyquinoline and C the antimony salt of 5-fiuoro-8-hydroxyqu o ine. In Table III, the products A, B and C have been compared with the reference product emetine hydrochloride (C.E.).

In Tables I, II and III, the number 0 and the signs and have the following significances:

0: total inhibited culture little plentiful culture plentiful culture very plentiful cuture.

I. Amoebicidal activity in vitro": inhibition at the start of the cultures Concentrations Product A 0 0 Product 13.... 0 Product C 0 II. Amoebicidal activity in vitro: inhibition at the start of the cultures Concentrations Product A 0 0 0 0 Product B 0 0 0 Product C 0 0 0 0 III. Amoebicidal activity "in vitro": lethal action on a culture of 3 days THEREPEUTIC APPLICATIONS l. The data for these different antimony salts of 8-hydroxyquinoline derivatives relate to the treatment of biharziosis, amoebiasis,.- leishmaniasis, filariasis, and

microbial diarrhoea. These products may be administered 5,7-dibromo-6-methyl-8-hydroxyquino1ine in the form of compressed tablets of 10 mg. each at the at a daily dose of 60 to 100 mg. of the salt per day. rate of 6 to 10 a day.

We claim: References Cited bei fii io riff "$31 533 i fi ii fifiniifi 5 UNITED STATES PATENTS s W c p lses 1 5 mg or y a 2,608,556 8/1952 Kalberg 424245 gi an f 'f ammmt of the ammo Salt 3,287,210 11/1966 Leebrick 424 .245

y roxyqumo me, 6-methyl-8-hydroxyquinoline, OTHER REFERENCES S-fluoro-8-hydroxyquinoline, 10 Chem. Abstracts, v01. 57 (1962), p. 9195 (d). 5-chloro-8-hydroxyquinoline, 5 nitm 3-hYdroXYcluinoline, ALBERT T. MEYERS, Primary Exammer y yq i i e v. D. TURNER, Assistant Examiner 5-chloro-7-iodo-8-hydroxyquinol1ne,

5,7-dibromo-8-hydroxyquinoline, 15 US. Cl. X.R. 5-chloro-7-iodo-6-methyl-8-hydroxyquinoline or 260289 R Y 

